Dnmt3a Rabbit Monoclonal Antibody
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產(chǎn)品編號(hào)
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產(chǎn)品名稱(chēng)
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY1732
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Dnmt3a Rabbit
Monoclonal Antibody
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50μl
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618.00元
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來(lái)源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IF, ICC
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H, R
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120.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測(cè)或其它適當(dāng)用途時(shí)的一抗稀釋。
建議抗體使用時(shí)的稀釋比例如下(實(shí)際使用時(shí)需根據(jù)抗原水平的高低作適當(dāng)調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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1:50-1:200
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-
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1:50-1:200
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-
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-
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-
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抗體詳細(xì)信息如下:
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About this
Antibody
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Name
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Dnmt3a Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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1788(Human);
444984(Rat)
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SwissProt
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Q9Y6K1(Human);
Q1LZ53(Rat)
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Synonyms
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DNMT3A2;
M.HsaIIIA; TBRS
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Category
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Epigenetics
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Background
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Methylation at
the 5'-position of cytosine is the only known naturally occurring covalent
modification of the mammalian genome. DNA methylation requires the enzymatic
activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze
the transfer of a methyl group from S-adenosyl methionine to the 5'-position
of cytosines residing in the dinucleotide CpG motif, and this methylation
results in transcriptional repression of the target gene. The Dnmt enzymes
are encoded by independent genes. Dnmt1 is the most abundant, and it
preferentially methylates hemimethylated DNA and coordinates gene expression
during development. Additional mammalian Dnmt proteins include Dnmt2 and
Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed
at substantially lower levels in ***** tissues, and is likely involved in
methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by
two distinct genes, but both are abundantly expressed in embryonic stem
cells, where they also methylate CpG motifs on DNA.
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包裝清單:
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產(chǎn)品編號(hào)
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產(chǎn)品名稱(chēng)
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包裝
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XY1732
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Dnmt3a Rabbit
Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說(shuō)明書(shū)
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1份
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保存條件:
Dnmt3a Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長(zhǎng)期不使用可以考慮-20℃保存，但凍融可能會(huì)導(dǎo)致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項(xiàng):
如果本抗體用于Western blot (WB)、**熒光(IF)、**細(xì)胞化學(xué)(ICC)等實(shí)驗(yàn)，請(qǐng)注意回收使用過(guò)的稀釋抗體。回收的抗體通常至少可以重復(fù)使用5-10次。稀釋后的抗體，包括已經(jīng)使用過(guò)的稀釋抗體，請(qǐng)4℃保存。
回收后重復(fù)使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復(fù)使用過(guò)程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測(cè)。如果回收的抗體出現(xiàn)明顯的絮狀物或長(zhǎng)霉長(zhǎng)菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細(xì)胞化學(xué)(ICC)等適當(dāng)用途。如果希望獲得*佳的檢測(cè)效果，請(qǐng)考慮使用上述檢測(cè)專(zhuān)用的一抗稀釋液。
本產(chǎn)品**于專(zhuān)業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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宋體;color:#666666;mso-font-kerning:0pt'> 為了您的**和健康，請(qǐng)穿實(shí)驗(yàn)服并戴一次性手套操作。
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