AMPK alpha 1 Rabbit Monoclonal Antibody
|
產品編號
|
產品名稱
|
產品包裝
|
產品價格
|
|
XY1627
|
AMPK alpha 1
Rabbit Monoclonal Antibody
|
50μl
|
618.00元
|
|
來源
|
用途
|
交叉反應性
|
分子量
|
|
Rabbit
|
WB, IP, IF,
ICC, FC
|
H, M, R
|
63.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
1:10-1:50
|
1:50-1:200
|
-
|
1:50-1:200
|
1:50-1:100
|
-
|
-
|
抗體詳細信息如下:
|
About this
Antibody
|
|
Name
|
AMPK alpha 1
Rabbit Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
5562(Human);
105787(Mouse); 65248(Rat)
|
|
SwissProt
|
Q13131(Human);
Q5EG47(Mouse); P54645(Rat)
|
|
Synonyms
|
PRKAA1; AMPK;
AMPKa1
|
|
Category
|
Cell Cycle
|
|
Background
|
AMPK (for
5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a
catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP
depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is
activated by high AMP and low ATP through a mechanism involving allosteric
regulation, promotion of phosphorylation by an upstream protein kinase known
as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can
phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and
acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis
and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid
and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino
acid proteins, respectively, contain unique N-terminal domains and may
participate directly in the binding of AMP within the AMPK complex.
|
包裝清單:
|
產品編號
|
產品名稱
|
包裝
|
|
XY1627
|
AMPK alpha 1
Rabbit Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀釋液
|
50ml
|
|
-
|
說明書
|
1份
|
保存條件:
AMPK alpha 1
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
- 溫馨提示：為規避購買風險，建議您在購買前務必確認供應商資質與產品質量。
- 免責申明：以上內容為注冊會員自行發布，若信息的真實性、合法性存在爭議，平臺將會監督協助處理，歡迎舉報