FOXO1A Rabbit Monoclonal Antibody
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價格
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XY1600
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FOXO1A Rabbit
Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應(yīng)性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H, M, R, Z
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80.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據(jù)抗原水平的高低作適當調(diào)整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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FOXO1A Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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2308(Human);
56458(Mouse); 84482(Rat)
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SwissProt
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Q12778(Human);
Q9R1E0(Mouse); G3V7R4(Rat)
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Synonyms
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FKH1; FKHR;
FOXO1A
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Category
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PI3K/Akt/mTOR
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Background
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FKHR (for
forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family
of transcription factors. Transcriptional activation of FKHR proteins is
regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1
and results in FKHRL1 associating with 14-3-3 proteins and being retained in
the cytoplasm. Induction of apoptosis or withdrawal of growth factors
stimulates dephosphorylation and nuclear translocation of FKHR proteins,
leading to FKHR-induced gene-specific transcriptional activation. FKHR, also
designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed
protein that shuttles between the cytoplasm and nucleus. Genetic mutations in
FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly
found in alveolar rhabdomyosarcomas. These translocations result in the
fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and
homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which
contains a transcriptional activation domain. The Pax-3/FKHR fusion protein
appears to function as an oncogenic transcription factor that enhances the
activation of normal Pax-3 target genes.
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY1600
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FOXO1A Rabbit
Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
FOXO1A Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現(xiàn)輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體?；厥盏目贵w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發(fā)現(xiàn)抗體出現(xiàn)輕微混濁現(xiàn)象，可以10,000g離心1-3分鐘，取上清用于后續(xù)檢測。如果回收的抗體出現(xiàn)明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產(chǎn)品**于專業(yè)人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
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