Cleaved PARP Rabbit Monoclonal Antibody
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY1567
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Cleaved PARP Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IP, IF, ICC, FC
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H
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89.0kDa
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WB, Western
blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC,
Immunohistochemistry; ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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1:10-1:50
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1:50-1:200
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-
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1:50-1:200
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1:50-1:100
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-
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-
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抗體詳細信息如下:
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About this Antibody
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Name
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Cleaved PARP Rabbit Monoclonal Antibody
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Category
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Rabbit Monoclonal Antibody (RabMAb);
Primary antibody
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Isotype
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IgG
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Purification
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XYfinity purification
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About the Immunogen
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Immunogen
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Recombinant protein
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Gene ID
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142(Human)
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SwissProt
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P09874(Human)
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Synonyms
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ADPRT; ADPRT 1; ADPRT1; ARTD1; PARP;
PARP-1; PPOL; pADPRT-1; PARP1
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Category
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Apoptosis
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Background
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Poly(ADP-ribose) polymerase-1 (PARP-1),
also designated PARP, is a nuclear DNA-binding zinc finger protein that
influences DNA repair, DNA replication, modulation of chromatin structure,
and apoptosis. In response to genotoxic stress, PARP-1 catalyzes the transfer
of ADP-ribose units from NAD(+) to a number of acceptor molecules including
chromatin. PARP-1 recognizes DNA strand interruptions and can complex with
RNA and negatively regulate transcription. Actinomycin D- and
etoposide-dependent induction of caspases mediates cleavage of PARP-1 into a
p89 fragment that traverses into the cytoplasm. Apoptosis-inducing factor
(AIF) translocation from the mitochondria to the nucleus is PARP-1-dependent
and is necessary for PARP-1-dependent cell death. PARP-1 deficiencies lead to
chromosomal instability due to higher frequencies of chromosome fusions and
aneuploidy, suggesting that poly(ADP-ribosyl)ation contributes to the
efficient maintenance of genome integrity.This antibody recognizes the
apoptosis-specific 89 kDa catalytic domain fragment, but it does not
recognize the full-length PARP-1 or the 24 kDa DNA binding domain fragment.
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY1567
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Cleaved PARP Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Cleaved PARP
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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