PI 3 Kinase Class 3 Rabbit Monoclonal Antibody
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY1549
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PI 3 Kinase
Class 3 Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IHC
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H, M, R
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100.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000
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-
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-
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1:50-1:200
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-
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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PI 3 Kinase
Class 3 Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5289(Human);
225326(Mouse); 65052(Rat)
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SwissProt
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Q8NEB9(Human);
Q6PF93(Mouse); O88763(Rat)
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Synonyms
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Phosphatidylinositol
3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3;
PtdIns-3-kinase type 3; Phosphatidylinositol 3-kinase p100 subunit; hVps34;
PIK3C3; VPS34
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Category
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Immunology/Inflamation;
Metabolism
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Background
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Phosphatidylinositol
3-kinases (PI3Ks) phosphorylate the 3' OH position of the inositol ring of
inositol lipids. PI 3-kinase p100 (phosphoinositide-3-kinase p100 subunit),
also known as hVps34 or PIK3C3 (phosphoinositide-3-kinase, class III), is a
member of the PI3/PI4-kinase family. It is ubiquitously expressed with
predominant expression in skeletal muscle and is believed to participate in
endosome to lysosome transport, multivesicular body formation, autophagy and
retrograde endosome to Golgi transport. PI 3-kinase p100 is the catalytic
subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory
subunit of class 3 PI3Ks. PI 3-kinase p100 exclusively phosphorylates
phosphatidylinositol to produce PtdIns3P. Unlike class I PI3Ks, whose activities
are enhanced in the presence of magnesium, PI 3-kinase p100 activity is
enhanced by manganese. Its activity can also be regulated by nutrients,
suggesting an important role of PI-3 kinase p100 in the regulation of mTOR
protein synthesis and autophagy.
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY1549
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PI 3 Kinase
Class 3 Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
PI 3 Kinase
Class 3 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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