Phospho-Akt1 (Ser473) Rabbit Monoclonal Antibody
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY1546
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Phospho-Akt1
(Ser473) Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H, M
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56.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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Phospho-Akt1
(Ser473) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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207(Human);
11651(Mouse)
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SwissProt
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P31749(Human);
P31750(Mouse)
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Synonyms
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AKT; CWS6;
PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
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Category
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PI3K/Akt/mTOR
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Background
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The
serine/threonine kinase Akt family contains several members, including Akt1
(also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated
PKBγ or thyoma viral proto-oncogene 3), which exhibit
sequence homology with the protein kinase A and C families and are encoded by
the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin
homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This
activation is dependent on PDGFR-β tyrosine residues
740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI
3-kinase) complex. Activation of Akt1 by insulin or insulin-growth
factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473.
Phosphorylation of both residues is important to generate a high level of
Akt1 activity, and the phosphorylation of Thr 308 is not dependent on
phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated
and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The
activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor
wortmannin, suggesting that the protein signals downstream of the PI kinases.
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY1546
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Phospho-Akt1
(Ser473) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Phospho-Akt1
(Ser473) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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