Topoisomerase II alpha Rabbit Monoclonal Antibody
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY1525
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Topoisomerase
II alpha Rabbit Monoclonal Antibody
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50μl
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618.00元
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來源
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用途
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交叉反應性
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分子量
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Rabbit
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WB, IP, IHC
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H, M, R
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174.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀釋液，可以用于Western檢測或其它適當用途時的一抗稀釋。
建議抗體使用時的稀釋比例如下(實際使用時需根據抗原水平的高低作適當調整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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-
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1:50-1:200
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-
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-
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-
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-
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抗體詳細信息如下:
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About this
Antibody
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Name
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Topoisomerase
II alpha Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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7153(Human);
21973(Mouse); 360243(Rat)
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SwissProt
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P11388(Human);
Q01320(Mouse); P41516(Rat)
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Synonyms
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DNA
topoisomerase 2-alpha; TOP2A; TOP2
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Category
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Cancer Biology
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Background
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DNA
topoisomerase I and II (Topo I and Topo II) are nuclear enzymes that regulate
the topological structure of DNA in eukaryotic cells by transiently breaking
and rejoining DNA strands. Eukaryotic topoisomerases are capable of relaxing
both positive and negative supercoils, whereas prokaryotic topoisomerases
relax only negative supercoils. DNA topoisomerases play a role in DNA
replication, recombination, and transcription and have been identified as
targets of numerous anticancer drugs. Topo I, a ubiquitously expressed,
soluble enzyme, acts by introducing a transient break in one strand of DNA,
while Topo II acts by making a transient double-strand break. Topo II is
encoded by two different genes to generate two distinct isoforms that are
designated Topo IIα and Topo IIβ. Topo IIβ and Topo IIα, are largely homologous at
their N-terminal three quarters, however, the C-terminal segments are
considerably divergent, suggesting that these regions may mediate different
cellular functions and account for the observed differential tissue
expression patterns of the two isoforms.
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY1525
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Topoisomerase
II alpha Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀釋液
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50ml
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-
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說明書
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1份
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保存條件:
Topoisomerase II
alpha Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優先推薦4℃保存，長期不使用可以考慮-20℃保存，但凍融可能會導致出現輕微的渾濁和少量不溶物。
注意事項:
如果本抗體用于Western blot (WB)、**熒光(IF)、**細胞化學(ICC)等實驗，請注意回收使用過的稀釋抗體。回收的抗體通常至少可以重復使用5-10次。稀釋后的抗體，包括已經使用過的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過程中發現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長霉長菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于**熒光(IF)、**組化(IHC)、**細胞化學(ICC)等適當用途。如果希望獲得*佳的檢測效果，請考慮使用上述檢測專用的一抗稀釋液。
本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。
為了您的**和健康，請穿實驗服并戴一次性手套操作。
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