Recombinant Human sRANK Receptor/TNFRSF11A
產品編號
產品名稱
產品包裝
產品價格
XY417-1mg
1mg
5665.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
8792
Q9Y6Q6
E. coli
174aa
19.1kDa
－
About this Protein
Name
Recombinant Human sRANK Receptor/TNFRSF11A (Recombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor; rHusRANK Receptor)；重組人可溶性核因子κB受體激活劑/TNFRSF11A
Synonyms
CD265; ODFR; TNFRSF11A; TRANCE R; CD265; CD265 antigen; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50ng/ml, corresponding to a specific activity of >2.0×104IU/mg in the presence of 15ng/ml of recombinant sRANK Ligand.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Endotoxin
Less than 0.1EU/μg of rHusRANK Receptor as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate na?ve T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cellmediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.1kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (174 amino acid residues).
Amino Acid Sequence
QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK
包裝清單:
包裝
XY417-20μg
20μg
XY417-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應濃度的儲存液后(請根據產品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。 本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。 為了您的**和健康，請穿實驗服并戴一次性手套操作。