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Background
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Angiostatin,
is a ~30kDa fragment of plasminogen that is encoded by the PLG gene in
humans. It is produced, for example, bybautoproteolytic cleavage of
plasminogen, involving extracellular disulfide bond reduction by
phosphoglycerate kinase.bFurthermore, angiostatin can be cleaved from
plasminogen by different metalloproteinases (MMPs), elastase,
prostate-specific antigen (PSA), 13kDa serine protease, or 24kDa
endopeptidase. Angiostatin is known to bind many proteins, especially to
angiomotin and endothelial cell surface ATP synthase but also integrins,
annexin II, C-met receptor, ng2 proteoglycan, tissuetype plasminogen
activator, chondroitin sulfate proteoglycans, and CD26. It seems to involve
inhibition of endothelial cell migration, proliferation and induction of
apoptosis, but its mechanism of action is still unclear. Angiostatin is
currently undergoing clinical trials for its use in anticancer therapy.
Recombinant angiostatin is expressed in E. coli.
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