Recombinant Human IL-1 RA
產(chǎn)品編號
產(chǎn)品名稱
產(chǎn)品包裝
產(chǎn)品價(jià)格
XY111-1mg
1mg
5665.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
3557
P18510
E. coli
152aa
17.1kDa
－
About this Protein
Name
Recombinant Human IL-1 RA (Recombinant Human Interleukin-1 Receptor Antagonist; rHuIL-1RA)；重組人白細(xì)胞介素1受體拮抗劑
Synonyms
IL-1F3; IL1RN; DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; Interleukin 1 receptor antagonist; IRAP; MVCD4
Purity
>96% by SDS-PAGE and HPLC analyses
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1α-dependent proliferation of murine D10S cells is less than 40ng/ml, corresponding to a specific activity of >2.5×104IU/mg in the presence of 50pg/ml rHuIL-1α.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
Endotoxin
Less than 1EU/μg of rHuIL-1RA as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Interleukin-1 receptor antagonist (IL-1RA) is a member of the IL-1 family. IL-1RA is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of IL-1β. This protein inhibits the activities of interleukin 1, alpha (IL-1α) and interleukin 1, beta (IL-1β), and modulates a variety of interleukin 1 related immune and inflammatory responses. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-RA. The regulated expression of IL-1RA in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1RA is markedly enhanced by IL-1, TNF-α, or PDGF. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α.
Amino Acid Sequence
RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
包裝清單:
包裝
XY111-20μg
20μg
XY111-100μg
100μg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。 注意事項(xiàng): 由于有些塑料管壁對某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。 本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。 為了您的**和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。