Recombinant Protein A/G, His-tag
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產(chǎn)品編號
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產(chǎn)品名稱
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產(chǎn)品包裝
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產(chǎn)品價(jià)格
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XY043-1g
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Recombinant
Protein A/G, His-tag
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1g
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38444.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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－
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－
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－
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E. coli
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513aa
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56.9kDa
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His
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About this
Protein
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Name
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Recombinant
Protein A/G, His-tag (Recombinant Protein A/G, His-tag; Protein A/G, His-tag)；重組蛋白A/G，His標(biāo)簽
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Synonyms
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N/A
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Purity
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>96% by
SDS-PAGE and RP-HPLC analyses.
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Biological
Activity
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The
recombinant Protein A/G is a genetically engineered protein containing 5
IgG-binding regions of protein A and 3 of protein G. Cell wall binding
region, cell membrane binding region and albumin binding region have been
removed from the recombinant Protein A/G to ensure the maximum specific IgG
binding. The recombinant Protein A/G is ideal for purification of polyclonal
or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and
rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b,
IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep,
house, rabbit, guinea pig, pig, dog and cat.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from additive free solution.
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Endotoxin
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Less than
0.1EU/μg of Protein A/G, His-tag as determined by LAL method.
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Reconstitution
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Dissolve in
distilled water or saline.
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Category
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Protein A/G
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Background
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The
recombinant Protein A/G consists of 8 IgG-binding domains EDABC-C1C2C3, which
corresponds to the Protein A and G domains that are included in the
recombinant sequence. The Protein A portion is from Staphylococcus aureus
segments E, D, A, B and C. The Protein G portion is from Streptococcus
segments C1, C2 and C3. The binding capacity of recombinant Protein A/G is
broader than either Protein A or Protein G alone.
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Amino Acid
Sequence
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MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG
FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP
SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK
LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK
FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI
LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV
INGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV
INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE KLAAALEHHH HHH
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包裝清單:
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產(chǎn)品編號
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產(chǎn)品名稱
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包裝
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XY043-1mg
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Recombinant
Protein A/G, His-tag
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1mg
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XY043-5mg
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Recombinant
Protein A/G, His-tag
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5mg
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XY043-1g
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Recombinant
Protein A/G, His-tag
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1g
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應(yīng)濃度的儲存液后(請根據(jù)產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復(fù)凍融。
注意事項(xiàng):
由于有些塑料管壁對某些蛋白有較強(qiáng)的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預(yù)先封閉塑料管壁上的蛋白結(jié)合位點(diǎn)，使細(xì)胞因子或重組蛋白不會粘附于管壁。所以一定要使用產(chǎn)品簡介中Reconstitution一欄的信息配制儲存液。
本產(chǎn)品**于專業(yè)人員的科學(xué)研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內(nèi)。
為了您的**和健康，請穿實(shí)驗(yàn)服并戴一次性手套操作。
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