Recombinant Cys-Protein G
產品編號
產品名稱
產品包裝
產品價格
XY5039-1g
1g
27246.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
－
E. coli
201aa
40kDa
About this Protein
Name
Recombinant Cys-Protein G (Recombinant Cys-Protein G; Cys-Protein G)；重組蛋白G，N端帶Cys
Synonyms
N/A
Purity
>95% by SDS-PAGE and RP-HPLC analyses.
Biological Activity
The recombinant Protein G is a genetically engineered protein containing 3 IgG-binding regions of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein G to ensure the maximum specific IgG binding. The recombinant Protein G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and rabbit.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from additive free solution.
Endotoxin
Less than 0.1EU/μg of Cys-Protein G as determined by LAL method.
Reconstitution
Dissolve in distilled water or saline.
Category
Protein G
Background
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
Amino Acid Sequence
CLPKTDTYKL ILNGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTEAVDAAT AEKVFKQYAN DNGVDGEWTY DDATKTFTVT EKPEVIDASE LTPAVTTYKL VINGKTLKGE TTTKAVDAET AEKAFKQYAN DNGVDGVWTY DDATKTFTVT E
包裝清單:
包裝
XY5039-1mg
1mg
XY5039-10mg
10mg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應濃度的儲存液后(請根據產品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。 本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。 為了您的**和健康，請穿實驗服并戴一次性手套操作。