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- 產品名稱:Recombinant Protein G, His-tag
- 產品型號:
- 產品展商:XYbscience
- 產品文檔:無相關文檔
簡單介紹
Recombinant Protein G, His-tag由于有些塑料管壁對某些蛋白有較強的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。Recombinant Protein G, His-tag而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點???使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。
產品描述
Recombinant Protein G, His-tag
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產品編號
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產品名稱
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產品包裝
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產品價格
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XY5030-1g
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Recombinant
Protein G, His-tag
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1g
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24768.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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-
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-
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-
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E. coli
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210aa
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29kDa
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His
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About this
Protein
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Name
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Recombinant
Protein G, His-tag (Recombinant Protein G, His-tag; Protein G, His-tag);重組蛋白G,His標簽
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Synonyms
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N/A
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Purity
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>97% by
SDS-PAGE and RP-HPLC analyses
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Biological
Activity
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The
recombinant Protein G is a genetically engineered protein containing 3
IgG-binding regions of protein G. Cell wall binding region, cell membrane
binding region and albumin binding region have been removed from the
recombinant Protein G to ensure the maximum specific IgG binding. The
recombinant Protein G is ideal for purification of polyclonal or monoclonal
IgG antibodies. Protein G binds to various human, mouse and rat IgG
subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat
IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house and
rabbit.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from additive free solution.
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Endotoxin
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Less than
0.1EU/μg of Protein G, His-tag as determined by LAL method.
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Reconstitution
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Dissolve in
distilled water or saline.
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Category
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Protein G
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Background
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Protein G is a
bacterial protein derived from the cell wall of certain strains of
b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of
various classes and subclasses of immunoglobulins from a variety of species.
Protein G binds to all IgG subclasses from human, mouse and rat species. It
also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse.
Protein G binds preferentially to the Fc portion of IgG, but can also bind to
the Fab region, making it useful for purification of F(ab') fragments of IgG.
Due to it's affinity for the Fc region of many mammalian immunoglobulins,
protein G is considered a universal reagent in biochemistry and immunology.
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Amino Acid
Sequence
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MGTYKLILNG KTLKGETTTE AVDAATAEKV
FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTE AVDAATAEKV
FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLKGETTTK AVDAETAEKA
FKQYANDNGV DGVWTYDDAT KTFTVTEKLA AALEHHHHHH
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包裝清單:
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產品編號
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產品名稱
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包裝
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XY5030-1mg
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Recombinant
Protein G, His-tag
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1mg
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XY5030-10mg
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Recombinant
Protein G, His-tag
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10mg
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XY5030-1g
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Recombinant
Protein G, His-tag
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1g
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-
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說明書
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1份
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保存條件:
-20℃或更低溫度保存,至少一年有效。由于蛋白的每次凍融均會引起部分失活,所以**配制成相應濃度的儲存液后(請根據產品簡介中Reconstitution一欄的信息配制儲存液),須分裝后-20℃或更低溫度凍存,以避免反復凍融。
注意事項:
由于有些塑料管壁對某些蛋白有較強的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein,如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點,使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。
本產品**于專業人員的科學研究用,不得用于臨床診斷或**,不得用于食品或藥品,不得存放于普通住宅內。
為了您的**和健康,請穿實驗服并戴一次性手套操作。
- 溫馨提示:為規避購買風險,建議您在購買前務必確認供應商資質與產品質量。
- 免責申明:以上內容為注冊會員自行發布,若信息的真實性、合法性存在爭議,平臺將會監督協助處理,歡迎舉報