Recombinant Protein A-Cys
產品編號
產品名稱
產品包裝
產品價格
XY5012-1g
1g
5735.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
－
E. coli
297aa
33.5kDa
About this Protein
Name
Recombinant Protein A-Cys (Recombinant Protein A-Cys; Protein A-Cys)；重組蛋白A，C端帶Cys
Synonyms
N/A
Purity
>98% by SDS-PAGE and HPLC analyses
Biological Activity
The recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from additive free solution.
Endotoxin
Less than 0.1EU/μg of Protein A-Cys as determined by LAL method.
Reconstitution
Dissolve in distilled water or saline.
Category
Protein A
Background
Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.
Amino Acid Sequence
NAAQHDEAQQ NAFYQVLNMP NLNADQRNGF IQSLKDDPSQ SANVLGEAQK LNDSQAPKAD AQQNNFNKDQ QSAFYEILNM PNLNEAQRNG FIQSLKDDPS QSTNVLGEAK KLNESQAPKA DNNFNKEQQN AFYEILNMPN LNEEQRNGFI QSLKDDPSQS ANLLSEAKKL NESQAPKADN KFNKEQQNAF YEILHLPNLN EEQRNGFIQS LKDDPSQSAN LLAEAKKLND AQAPKADNKF NKEQQNAFYE ILHLPNLTEE QRNGFIQSLK DDPSVSKEIL AEAKKLNDAQ APKEEDC
包裝清單:
包裝
XY5012-10mg
10mg
XY5012-100mg
100mg
-
說明書
1份
保存條件: -20℃或更低溫度保存，至少一年有效。由于蛋白的每次凍融均會引起部分失活，所以**配制成相應濃度的儲存液后(請根據產品簡介中Reconstitution一欄的信息配制儲存液)，須分裝后-20℃或更低溫度凍存，以避免反復凍融。 注意事項: 由于有些塑料管壁對某些蛋白有較強的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很難與管壁分離。而載體蛋白(Carrier protein，如0.1% BSA等)的主要作用是預先封閉塑料管壁上的蛋白結合位點，使細胞因子或重組蛋白不會粘附于管壁。所以一定要使用產品簡介中Reconstitution一欄的信息配制儲存液。 本產品**于專業人員的科學研究用，不得用于臨床診斷或**，不得用于食品或藥品，不得存放于普通住宅內。 為了您的**和健康，請穿實驗服并戴一次性手套操作。